The Hemoglobinopathy Fractionation Profile Blood Test, or Hemoglobin Fractionation, aids in the diagnosis of hemoglobinopathy, an inherited blood disorder characterized by the presence of an abnormal form of hemoglobin (known as a variant) or decreased hemoglobin production (known as thalassemia). Hemoglobin (Hb) is a protein found in red blood cells (RBCs) that binds to oxygen and helps transport it throughout the body. The shape, size and function of RBCs may be altered when hemoglobin variants are present, which could result in inefficient oxygen transportation and premature destruction of the cells, leading to hemolytic anemia. Symptoms of hemolytic anemia include weakness, fatigue, jaundice and pale skin. Severe hemoglobinopathies, such as homozygous Hb S (Sickle Cell Anemia), can result in symptoms such as episodes of acute pain, shortness of breath and an enlarged spleen. Hemoglobin Fractionation quantifies (in relative percentages) the normal forms of hemoglobin present, including A, A2 and F, as well as the variants S and C. Percentages present in normal adults are as follows: •Hb A: 94-98% •Hb A2: 0.7% to 3.1% •Hb F: Less than 2% •Hb S: 0% •Hb C: 0% Hemoglobin Fractionation is also known as Hb Electrophoresis Plus Solubility. No fasting is required prior to this blood test.